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KMID : 0364320190440010009
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Journal of East-West Medicine
2019 Volume.44 No. 1 p.9 ~ p.16
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Processing effect on cytoprotective activity of Gardeniae Fructus in gastric epithelial cells induced by tert-butyl hydroperoxide
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Park Sook-Jahr
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Abstract
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Objectives : Gardeniae Fructus is the dried mature fruit of Gardenia jasminoides Ellis that has been used as a Korean medicine for treating digestive disorders, liver disease and various inflammatory disorders. In this study, we aimed to evaluate the processing effect on protective activity of Gardeniae Fructus in tBHP-induced AGS gastric epithelial cells.
Methods : The processed Gardeniae Fructus was made by adding ginger juice and alcohol to the Gardeniae Fructus or by roasting Gardeniae Fructus at 150℃ and 250℃ for 5, 10, and 20 minutes. The antioxidant activity was examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,20-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) assays. Cytoprotective activity was examined using MTT assay after treating cells with GFE and tert-butyl hydroperoxide (tBHP). To determine acid neutralizing activity, the excess HCl was immediately titrated with 0.1 N NaOH (end point pH 3.5).
Results : The antioxidative capacity of processed Gardeniae Fructus was higher than that of untreated Gardeniae Fructus. Cytoprotective and acid neutralizing activities were also higher in the processed Gardeniae Fructus than in the untreated Gardeniae Fructus. Compared to 57.8% of untreated Gardeniae Fructus, the processed Gardeniae Fructus containing 40% ginger juice and 10% rectified alcohol showed 64.3% and 69.3% of cell viability, respectively. Gardeniae Fructus, roasted at 150 ℃ for 5 minutes, had the highest cell protection effect with a cell viability of 77%.
Conclusions : These results show that proper processing conditions of Gardeniae Fructus may be beneficial in improving the cell protection effect of Gardeniae Fructus in tBHP-induced gastric epithelial cells.
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KEYWORD
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Gardeniae Fructus, Processing, antioxidative activity, tBHP, gastric cells, acid neutralizing activity
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